Journal: Cell Reports Medicine

Article Title: Trafficking and persistence of alloantigen-specific chimeric antigen receptor regulatory T cells in Cynomolgus macaque

doi: 10.1016/j.xcrm.2022.100614

Figure Lengend Snippet:

Article Snippet: Goat α-Mouse IgG F(ab) 2 -AF488 , Jackson Immunoresearch , Cat#114-454-072.

Techniques: Recombinant, Activation Assay, Enzyme-linked Immunosorbent Assay

Journal: Journal of Virology

Article Title: Protection of Newborn Macaques by Plant-Derived HIV Broadly Neutralizing Antibodies: a Model for Passive Immunotherapy during Breastfeeding

doi: 10.1128/JVI.00268-21

Figure Lengend Snippet: Production of anti-IgG1 Fc antibody in neonates injected with PGT121 or with a PGT121-LS+VRC07-LS cocktail. Newborns received 5 mg/kg of PGT121 s.c. (left) or 5 mg/kg each of PGT121-LS and VRC07-523-LS (right). ELISA plates were coated with PGT121-LS (purple), VRC07-LS (green), 1080 human Fc (CH1-CH2-CH3 domains) (blue), 1082 IgG1 Fc (CH2-CH3 domains only) (red), and CHO PGT121 (pale blue). Individual animal numbers are indicated at the top of each box. PGT121 (A) was injected weekly at weeks 0, 1, 2, 3, 4, and 5, while the cocktail (B) was injected at weeks 0, 2, and 4.

Article Snippet: Plates were washed 3 times with PBS with Tween 20 (PBST), blocked with 5% (wt/vol) milk in PBST for 2 h, washed 3 times, incubated for 2 h with monkey plasma or serum samples at 1/500 and 1/2,000 dilutions, washed 3 times, and incubated for 2 h with a 1/4,000 dilution of a peroxidase-labeled human-specific IgG1 Fc (SouthernBiotech).

Techniques: Injection, Enzyme-linked Immunosorbent Assay

Journal: Infection and Immunity

Article Title: Transmission-reducing and -enhancing monoclonal antibodies against Plasmodium vivax gamete surface protein Pvs48/45

doi: 10.1128/iai.00374-23

Figure Lengend Snippet: Competition between mAb 47 and mAb 5.55.5 for binding to Pvs48/45 in an ELISA. Panel (A) shows binding of HRP-conjugated mAb 5.55.5 in the presence of increasing ratio of unlabeled mAb 47 (dotted line) or unlabeled mAb 5.55.5 (solid line). Panel (B) shows binding of HRP-conjugated mAb 47 in the presence of increasing molar ratio of unlabeled mAb 5.55.5 (dotted line) or unlabeled mAb 47 (solid line). The assays were done in duplicate wells and repeated two times and the panels show data from a representative experiment.

Article Snippet: For IgG subtype analysis, primary antibodies were tested as above except that various IgG isotype-specific HRP-conjugated secondary antibodies were used (anti-mouse IgG1, IgG2a, IgG2b, and IgG3 from Southern Biotech, Birmingham, AL) followed by color development.

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay

Journal: Infection and Immunity

Article Title: Transmission-reducing and -enhancing monoclonal antibodies against Plasmodium vivax gamete surface protein Pvs48/45

doi: 10.1128/iai.00374-23

Figure Lengend Snippet: Binding of HRP-mAb 47 and HRP-mAb 5.55.5 to Pvs48/45 in an ELISA in the presence of excess of all the mAbs. All the competing unlabeled mAbs identified on x-axis were tested at 25- to 50-fold excess concentration. Panel (A) shows binding of HRP-conjugated mAb 5.55.5 in the presence of unlabeled competing mAbs. Panel (B) shows binding of HRP-conjugated mAb 47 in the presence of competing unlabeled mAbs. Figure shows results with 50-fold excess of competing mAbs with similar pattern revealed when competing mAbs were tested at 25-fold molar excess.

Article Snippet: For IgG subtype analysis, primary antibodies were tested as above except that various IgG isotype-specific HRP-conjugated secondary antibodies were used (anti-mouse IgG1, IgG2a, IgG2b, and IgG3 from Southern Biotech, Birmingham, AL) followed by color development.

Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Concentration Assay

Journal: bioRxiv

Article Title: ARGX-119, a therapeutic agonist antibody targeting MuSK

doi: 10.1101/2024.07.18.604166

Figure Lengend Snippet: (A) Binding of ARGX-119 or Ig1-like domain binding antibody control (3F6c-hIgG1-LALAdelK) to MuSK-ECD or MuSK Fz-like domain on ELISA. (B) SPR binding of ARGX-119 to MuSK. CM5 chip was coated with anti-human IgG-Fc and ARGX-119 was captured at low densities. Sensorgrams show the binding kinetics of 10 nM human, NHP, mouse and rat MuSK using a dissociation pH of 7.4 or 5.5. Kinetic parameters (C) ITC experiment, investigating the binding thermodynamics of ARGX-119 to human, NHP, mouse and rat MuSK in solution.

Article Snippet: Goat anti-human IgG antibodies and the Fcγ fragment specific (109-005-098, Jackson Immunoresearch) were covalently immobilized onto all the flow cells of a Sensor Chip CM5 Series S (Cytiva) at high density using amine coupling chemistry.

Techniques: Binding Assay, Control, Enzyme-linked Immunosorbent Assay